Wednesday, May 8, 2019

Green Fluorescent Protein Essay Example | Topics and Well Written Essays - 3250 words

Green kindling Protein - Essay ExampleThis generates a plethora of chemical transformations where glycine forms a chemical bond with serine, as a result a new closed ring is formed, and the process is followed by dehydration. In the near hour, environmental oxygen reacts with the bond in tyrosine resulting in the formation of double bond to form a new fluorescent chromophore. GFP is ideal for genetic engineering as it has its own chromophore. Genetic instructions argon provided to the cell under investigation, which eventually builds the GFP protein, GFP folds up in a specific manner and glows. Various investigate studies involve GFP as a reporter molecule to understand the functioning of genes or molecules in an diaphanous manner.Green Fluorescent Protein (GFP) is a protein molecule generated by a jellyfish Aequorea victoria. The organism displays its fluorescent protein on the margin of its umbrella as the glowing points of light. The light is generated from the yellow tissue masses encompassing rough 6000-7000 photogenic cells. These photogenic cells produce light by means of bioluminescence, encompassing a calcium-activated photoprotein, popularly know as aequorin that produces green fluorescent protein (GFP) and blue-green light. GFP accepts energy from the protein molecule aequorin and gives out as green light (Green Fluorescent Protein, n.d.).GFP comprises of 238 amino acids in its protein. The protein remains highly stable in neutral buffers even at the temperature of 65C. GFP is also stable at wider pH range from 5.5 to 12. The protein molecule of GFP is strongly fluorescent, with a quantum say-so of about 80 percent as well as displays molar extinction coefficient of 2.2 x 104 cm-1 M-1. maximal fluorescence displayed by GFP is at 400 nm while lesser dot is displayed at 475nm, whereas the fluorescence emission peak is displayed at 509nm. The intrinsic fluorescence of the GFP is attributed to its exclusive covalently bound chromophore, which i s

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